主要特点:
多达 6 个荧光检测通道,允许进行多重 PCR。
有效降低多色串扰和边缘效应,无需ROX校正,减少样品和试剂用量
创新的扫描方式和时间分辨信号分离技术,提高检测灵敏度
独特的边缘温度补偿技术,最大限度减少“边缘效应”
用户友好的软件
采用长效 LED 灯的创新技术提供可靠性结果
主要特点:
多达 6 个荧光检测通道,允许进行多重 PCR。
有效降低多色串扰和边缘效应,无需ROX校正,减少样品和试剂用量
创新的扫描方式和时间分辨信号分离技术,提高检测灵敏度
独特的边缘温度补偿技术,最大限度减少“边缘效应”
用户友好的软件
采用长效 LED 灯的创新技术提供可靠性结果
Temperature control system | |
Sample capacity | 96 |
Reaction volume | 10-50 μl |
Thermal cycle technology | Peltier |
Max. Heating/Cooling rate | 6.0° C/s |
Heating temperature range | 4 – 100 °C |
Temperature accuracy | ± 0.2°C |
Temperature uniformity | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
Temperature gradient setting range | 30–100°C |
Temperature gradient difference setting range | 1 – 36°C |
Detection system | |
Excitation light source | 4/6 monochrome high efficiency LEDs |
Detection device | PMT |
Detection mode | Time-resolved signal separating technology |
Excitation/detection wavelength range | 455-650nm/510-715nm |
Fluorescent channels | 4/6 channels |
Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
Sensitivity | Single copy gene |
Resolution | 1.33 folds copy number difference can be distinguished in single-plex QPCR |
Dynamic range | 10 orders of magnitude copies |